【直送】ELK Biotechnology Mouse TPI1(Triosephosphate Isomerase 1) ELISA Kit 96T 1個 ELK6883

●Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triosephosphate Isomerase 1(TPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triosephosphate Isomerase 1(TPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triosephosphate Isomerase 1(TPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Triosephosphate Isomerase 1(TPI1) in the samples is then determined by comparing the OD of the samples to the standard curve.●サイズ：96T●Alternative Names：TIM; Triose-phosphate isomerase●Species：Mouse●Assay Type：Sandwich●Sensitivity：0.065 ng/mL●Standard：10 ng/mL●Detection range：0.16-10 ng/mL●Sample type：Serum, plasma, tissue homogenates and other biological fluids.●Assay length：4.5h●Research Area：●この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）